In the middle of the Fourth shinobi world war and after the death of Uchiha Itachi finally the two brothers faced each other.Itachi were searching for Kabuto to stop the "edo tensei" when Sasuke noticed him. Sasuke trying to confirm at first if that was his brother. Sasuke used his susanoo but Itachi countered it by his susanoo too at that point Sasuke confirmed that was his brother. Itachi was amazed because Sasuke can used susanoo also. Itachi ignored all the questions of his brother and try to escaped but Sasuke tried to follow him until Itachi found the hide out of Kabuto. Itachi said that things would be hard from now because Sasuke has also there.

 

Madara noted that Tsunade is slower than the raikage but she is stronger than the raikage. Madara admitted that Hashirama senju was more stronger than him and compared Hashirama from his grand daughter Tsunade that Hashirama can heal his wound without forming a hand seal. But Tsunade told Madara that the "will of fire" made Tsunade more stronger.



Tsunade set four  rules for all medical ninja such as;

1. Medical ninja shall never give up on treating their team mates as long as they can still breath.

2. Medical ninjas are not allowed to engage in battles.

3. Medical ninja shall be the last one to die in the team.

4. Only those who have mastered the "Byakugou" the (mitotic regeneration jutsu) are only allowed to break those above rules.


Tsunade performing his Byakugou.

With the help of other kages Madara was sealed by Gaara by his "Ultimate sand storm funeral". But  it was only the mokuton clone jutsu by Hashirama and at the end Tsunade was piercedby Madara.


Tsunade was pierced.


to show her medical ninjutsu Tsunade purposely open her guard to show to
Madara Uchiha that she can heal her self without performing any seals.


Madara used (Tajuu Mokuton Bunshin No Jutsu) known as multiple shadow clones to surely defeat the five kages.